Journal: British Journal of Pharmacology

Article Title: Newly developed serine protease inhibitors decrease visceral hypersensitivity in a post‐inflammatory rat model for irritable bowel syndrome

doi: 10.1111/bph.14396

Figure Lengend Snippet: TaqMan primers used for qPCR analysis of colonic and DRG samples

Article Snippet: Rabbit anti‐PAR4 was purchased from Alomone Labs. Rabbit anti‐PAR2 was obtained from Santa Cruz Biotechnology.

Techniques:

Journal: British Journal of Pharmacology

Article Title: Newly developed serine protease inhibitors decrease visceral hypersensitivity in a post‐inflammatory rat model for irritable bowel syndrome

doi: 10.1111/bph.14396

Figure Lengend Snippet: Immunohistochemical localization of PAR2, PAR4 and TRPA1 channels in sensory nerve fibres of the distal colon. (A–C) Representative images showing co‐localization of PAR2 (red) in CGRP‐immunopositive nerve fibres (green). (D–F) Representative images showing the presence of PAR4 immunoreactivity (red) in the colonic epithelium and in enteric nerve plexuses (arrowheads) but not in the CGRP‐immunoreactive nerve fibre population (inset). (G–I) Representative images showing co‐localization of TRPA1 protein (red) in CGRP‐immunopositive nerve fibres (green).

Article Snippet: Rabbit anti‐PAR4 was purchased from Alomone Labs. Rabbit anti‐PAR2 was obtained from Santa Cruz Biotechnology.

Techniques: Immunohistochemical staining

Journal: British Journal of Pharmacology

Article Title: Newly developed serine protease inhibitors decrease visceral hypersensitivity in a post‐inflammatory rat model for irritable bowel syndrome

doi: 10.1111/bph.14396

Figure Lengend Snippet: Relative mRNA expression of PARs and TRP channels in samples from colons, DRG T13‐L2 and DRG L6‐S1

Article Snippet: Rabbit anti‐PAR4 was purchased from Alomone Labs. Rabbit anti‐PAR2 was obtained from Santa Cruz Biotechnology.

Techniques: Expressing

Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: A novel pathogenetic factor of laryngeal attack in hereditary angioedema? Involvement of protease activated receptor 1

doi: 10.1186/s13223-022-00699-7

Figure Lengend Snippet: Parameters of primary antibodies used in the study

Article Snippet: PAR4 Rabbit anti-Human Polyclonal (C-Terminus) Antibody, 1:100 , LifeSpan BioSciences , LS-A1311-50.

Techniques:

Journal: PLoS ONE

Article Title: Calcium Mobilization And Protein Kinase C Activation Downstream Of Protease Activated Receptor 4 (PAR4) Is Negatively Regulated By PAR3 In Mouse Platelets

doi: 10.1371/journal.pone.0055740

Figure Lengend Snippet: Flow cytometric analysis of PAR4 expression in wild type (WT) (black line), PAR3 −/− (gray line), and PAR4 −/− (shaded) mice platelets using anti-PAR4-FITC antibodies.

Article Snippet: The anti-PAR4-FITC antibody was purchased from Alamone Labs Ltd. (Jerusalem, Israel).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Calcium Mobilization And Protein Kinase C Activation Downstream Of Protease Activated Receptor 4 (PAR4) Is Negatively Regulated By PAR3 In Mouse Platelets

doi: 10.1371/journal.pone.0055740

Figure Lengend Snippet: The HEK293 cells were transfected with: ( A ) PAR4-Luc (1 µg) and PAR3-GFP (0–2.5 µg), ( B ) PAR3-Luc (1 µg) and PAR3-GFP (0–2.5 µg), or ( C ) PAR4-Luc (1 µg) and PAR4-GFP (0–2.5 µg). As a control experiment, the HEK293 cells were transfected with: ( D ) PAR3-Luc (1 µg) and rho-GFP (0–0.12 µg), or ( E ) PAR4-Luc (1 µg) and rho-GFP (0–0.12 µg). Forty-eight hours post-transfection, the cells were analyzed for GFP expression, Luc expression, and BRET. The curves were plotted as the ratio of GFP to Luc and all points from 3–6 independent experiments were analyzed by global fit to a hyperbolic or linear curve. The surface expression of PAR3 and PAR4 in the HEK293 cells was determined by flow cytometry. ( G ) V5-PAR4-GFP and V5-PAR3-GFP were detected with a V5 tag antibody conjugated to Alexa Fluor 647. ( H ) HA-PAR4-LUC and HA-PAR3-LUC were detected with a HA tag antibody conjugated to Alexa Fluor 647. The results are the mean (± SD) of two independent experiments. The number of PAR4 and PAR3 molecules on the HEK293 cells surface is calculated from the V5 or HA antibody standard curve using quantitative flow cytometry ( F ).

Article Snippet: The anti-PAR4-FITC antibody was purchased from Alamone Labs Ltd. (Jerusalem, Israel).

Techniques: Transfection, Control, Expressing, Flow Cytometry